CareLit Fachartikel
Cryopreservation of Hepatic Stellate Cells: A Valid Method for Banking Progenitor Cells of Liver Fibrogenesis
R Weiskirchen, S Neyzen, E Van de Leur, AM Gressner · Zeitschrift für Gastroenterologie · 2005 · Heft 01
Dokument
852063
CareLit-ID
Jahr
2005
Publikation
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Zusammenfassung
Hepatic stellate cells (HSC) reside in the space of Disse and constitute 1.4% of the total liver volume and 5% of liver cell numbers [1]. Methods for isolating and maintaining HSC in culture are well documented and consistently high-quality cells have been successfully isolated from livers of many species [2]. When quiescent, HSC possess multiple intracytoplasmatic lipid droplets containing vitamin A and upon culturing on uncoated plastic these cells transdifferentiate into a myofibroblastic phenotype (MFB) representing a valuable in vitro model to investigate the processes involved in liver fibrogenesis [3]. Fu…
Schlagworte
apoptosis
cryoconservation
fibrogenesis
hepatic stellate cells
Cells
Liver
These
Isolated
Between
Cryopreservation
Methods
Furthermore
There
Different
Allowing
Addressing
