Breaking Immune Tolerance in Chronic Hepatitis B by Dendritic Cell Vaccination in HBV Trimera Mice
R Vuyyuru, F Rahman, S Schmitt, J Köck, M Geisler, D Strand, P Galle, WO Böcher · Zeitschrift für Gastroenterologie · 2005 · Heft 01
Bibliografische Angaben
Zusammenfassung
Introduction: Whereas spontaneous resolution from hepatitis B is associated with strong antiviral T cell responses, only weak immune responses are found in patients with chronic infection. Thus, induction of strong HBV-specific T cell reactivities might represent a new therapeutic strategy. Dendritic cells (DC) are highly specialised antigen presenting cells (APC) and might therefore serve as an effective therapeutic vaccine. Methods: DC were generated from MACS -seperated monocytes of patients and healthy cnotrols by incubation in IL–4 and GM-CSF. Apoptosis of HepG2 cells transfected with a 1.3 overlength HBV p…
