Detection of DCIS and invasive ductal carcinoma of the breast in human serum by protein profiling (SELDI)

Introduction: Several methods are suited to classify different cancers by testing RNA or protein expression. No method is available to screen for DCIS. This study is performed to differentiate serum samples of healthy women and patients with benign, premalignant and malignant lesions by protein profiling. Additionally the diagnostic value of SELDI-MS (surface-enhanced laser desorption ionisation- mass spectroscopy) for detection of DCIS and invasive ductal carcinoma is investigated. Methods: SELDI is a protein chip based procedure and is used for quantitative measurement of protein mixtures like serum. Here a we…