Evaluation of gut mucosal diamine oxidase activity (DAO) in patients with food allergy and ulcerative colitis, idiopathic ulcerative colitis and Crohn's Disease

An accumulation of histamine and mast cells is found in IBD and gastrointestinal food allergy. Histamine may exert a broad range of proinflammatory and immuno-regulatory effects, particularly if this amine isn't degraded enzymatically within short time. In this study the enzyme activity of diamine oxidase (DAO), one of the main histamine catabolizing enzymes, was investigated in patients with food allergy and ulcerative colitis, idiopathic ulcerative colitis and Crohn's Disease. The enzyme activity was determined in the homogenate of the biopsy using 14C-putrescine (substrate) during 60 minutes. The 14C labelled…