CareLit Fachartikel
Endocannabinoid Degradation and Oxidative Defense Mechanisms Determine Anandamide-induced Cell Death in Liver Cell Populations
SV Siegmund, E Seki, H Uchinami, BF Cravatt, DA Brenner, RF Schwabe · Zeitschrift für Gastroenterologie · 2006 · Heft 01
Dokument
854315
CareLit-ID
Jahr
2006
Publikation
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Metadaten
DOI
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Zusammenfassung
Background: We have shown that the endogenous cannabinoid anandamide (AEA) induces reactive oxygen species (ROS)-dependent cell death in primary hepatic stellate cells (HSCs), but not in primary hepatocytes. Aim: To determine cellular factors that control AEA-induced cell death in hepatic cell populations. Methods: Fatty acid amide hydrolase (FAAH) expression was analyzed by western blot and real time PCR in primary rat hepatocytes and primary rat HSCs. FAAH activity was determined colorimetrically. Cell death was analyzed by LDH release, propidium iodide uptake and western blot for caspase 3- and PARP cleavage.…
Schlagworte
Anandamide
Anti-Fibrotic Therapy
Cell Death
Endocannabinoids
Hepatic Stellate Cells
Hepatocytes
Zeitschrift für Gastroenterologie
