TGF-β enhances alcohol dependent hepatocyte damage via downregulation of alcohol dehydrogenase I

The adverse effect of alcohol in liver is mediated via interactions involving oxidative/non-oxidative metabolism, fat deposition and fibrogenic cytokines. This study assessed cross-talk between ethanol and TGF-β signaling in hepatocytes and its contribution to liver damage. To obtain a comprehensive view on TGF-β effects in hepatocytes, an Affymetrix microarray analysis was performed. Results were confirmed by qRT-PCR, IHC and Western blot. Cell toxicity was determined by lactate dehydrogenase assay. Neutral lipid deposition in hepatocytes was evaluated by OilRed-O staining. Small interfering RNA technique was u…