Human amnion cell culture: current limitations and potential for regenerative medicine

Aims: The identification of human amnion cells capable to acquire different cell fates in vitro, has provided exciting prospects for cell-based regenerative medicine. We focus on side-by-side analysis of amnion epithelial (AEC) and mesenchymal cell (AMC) for their vitality, expandibility and ‘stemness profilersquor; after isolation and under culture. Materials and Methods: AEC and AMC were isolated from 27 term placentas. Cell number, morphology, size, proliferation and apoptosis were analysed. Cells were characterized by flow cytometry, immunocytochemistry and RT-PCR for expression of immunologic and stem cell…