Peripheral blood gene expression markers of local colorectal diseases determined by whole genome mRNA array analysis
B Molnár, O Galamb, B Galamb, N Solymosi, F Sipos, S Spisák, K Tóth, P Miheller, T Zágoni, A Németh, Z Tulassay · Zeitschrift für Gastroenterologie · 2007 · Heft 05
Bibliografische Angaben
Zusammenfassung
Background and Aims: Gene expression analysis of peripheral blood using high-density oligonucleotide microarray can contribute to the determination of distant blood markers of local pathophysiological alterations in colorectal diseases. Methods: 8ml peripheral blood was collected, immediately lysed, stabilised in PAXGENE tubes. Next, total RNA was extracted, amplified after globin reduction and biotinylated from 8 patients with colorectal cancer, 8 with villous adenoma (>1cm), 8 with active IBD and 8 normal controls. Genome-wide gene expression profile was evaluated by HGU133Plus2.0 microarrays. RMA background c…
