Recombinant immunoblot for assessment of intrathecally synthesised paraneoplastic antineuronal antibodies in cerebrospinal fluid from patients with paraneoplastic neurological syn…

Background: Beside of pathophysiological interest, estimation of intrathecal synthesis (IS) of paraneoplastic antineuronal antibodies may increase the diagnostic sensitivity in rare cases of patients with clinically suggestive paraneoplastic neurological syndromes (PNS) and negative or uncertain results in serum. Detection of antigen-specific oligoclonal bands (OCB) in CSF seems to be the most sensitive marker of IS. However, this technique is not suitable for routine laboratory testing. Patients and methods: This study evaluates the suitability of a commercial immunoblot (ravo PNS-blot) employing recombinant pa…