Activation and polarization of T-helper cells depends on the dosage of LPS used as a danger signal for activation of dendritic cells

Although several studies have focused on allergic sensitization by dendritic cells, to date it is still open under which conditions these antigen presenting cells are able to induce an allergic immune response. Therefore we examined whether dendritic cells generated from bone marrow in the presence of GMCSF (BMDC) and pulsed with LPS-free allergen are able to induce an allergic immune response or further activation by a danger signal is required. Our study reveals that BMDC pulsed with LPS-free ovalbumine did not induce an allergic immune response. However, when BMDC were activated with low dose LPS during pulsi…