In-vitro cell confluency affects primary human dermal fibroblast sensitivity to low concentrations of t-butylhydroperoxide

Introduction: Mitochondria play a central role in ATP production, radical oxygen species (ROS) generation, calcium signaling and apoptosis. Oxidative stress derived from mitochondria is associated with intrauterine growth restriction (IUGR), cardiovascular disease, cancer and aging. The molecular link between oxidative stress and these conditions is, however, poorly understood. Interestingly, elevated levels of intracellular S-adenosylhomocysteine (SAH), a potent inhibitor of most cellular methyltransferases, is also associated with IUGR, cardiovascular disease, cancer and aging. The molecular mechanism underlyi…