Effects of normal human insulin, insulin aspart, and insulin detemir on the lifespan of Caenorhabditis elegans

Aims: The nematode C. elegans possesses an insulin receptor which is highly homologues to the human insulin receptor and is able to bind human insulin. Hyperglycaemic culture conditions of C. elegans lead to reduction of lifespan, neuronal damage, and impairment of motility. Aim of this project was to study the effects of normal human insulin and the insulin analogues insulin aspart and insulin detemir under normal and hyperglycaemic conditions, with respect to life span and neuronal function. Methods: Wild type C. elegans were cultivated under standard and hyperglycaemic conditions, without insulin and in the p…