Beyond the adipose tissue – granulocytes as an additional source of circulating NAMPT in obesity

NAMPT has been proposed as an adipocytokine that is involved in the regulation of glucose homeostasis. We recently found that NAMPT was independently associated with leukocyte number. Therefore, we analysed in this study NAMPT production among several tissues and leukocyte subpopulations and assessed whether NAMPT is enzymatically active in cell lysates and supernatants. First, we examined NAMPT mRNA expression in 12 different tissues (adipose tissue, aorta, brain, heart, hypothalamus, kidney, liver, pancreas, peripheral blood leukocytes, skeletal muscle, smooth muscle, spleen). The expression of NAMPT was more…