The DNA-binding protein YB-1 is a direct MYCN target and influences repair and resistance in neuroblastoma cell lines

YB-1 regulates both gene transcription and translation, and increased YB-1 expression and nuclear localization has been associated with aggressive cancer phenotypes. We examined YB-1 expression, assessed the effect of YB-1 knockdown on cell viability and repair capacity and transcriptional control via MYCN in neuroblastoma (NB) cell models. YB-1 was expressed in NB cell lines, and expression was elevated in a cisplatin-resistant cell line. Cisplatin treatment induced YB-1 expression and nuclear translocation, and YB-1 knockdown increased sensitivity to cisplatin and the number of γH2AX foci, indicating reduced r…