Genotype analysis, source tracing and control strategy of an outbreak of carbapenem-resistant Acinetobacter Baumannii in a NICU

Aims: An outbreak of 17 cases of carbapenem-resistant acinetobacter baumannii in a neonate intensive care unit (NICU) was analyzed and evaluated. Methods: Samples were isolated from blood or sputum from the patients in the NICU, cultured by conventional techniques and automated system. Multiplex PCR was used to detect blaOXA-51-like, blaOXA-23-like, OXA-24, OXA-58 and Ambler class B carbapenemases. The genotype of the outbreak strains were identified by pulsed-field gel electrophoresis (PFGE). Results: BlaOXA-23 was detected in the isolates. 14 of the 17 isolates displayed similar genotype with the isolates from…