Protein protein interaction of p53 and p73 with YY1 in human hepatocellular carcinoma

Introduction: We have previously described a relevant role of the p53 family members in tumorigenesis, therapeutic response and prognosis of hepatocellular carcinoma (HCC). Since protein interaction networks play a key role in signaling pathways of different tumor entities we used the LUMIER-assay (LUminescence-based Mammalian IntERactome mapping) which is a high-throughput technology to identify protein protein interactions of the p53 family in HCC. Methods and results: First we established the LUMIER-assay in Hep3B cells to be able to map protein interaction networks in liver cells and apply it to the p53 and…