Clonal tracking of cells in the liver by multicolour RGB-Marking

To investigate regeneration of healthy liver and the development of cancer, understanding of the clonal composition of tissues is necessary. Knowledge of the clonal behaviour of regenerating liver cells and tumour cells reveals insights into the organ’s constitution on the single cell level. Only few and complex methods are available to distinguish cells and clones. As an alternative, stable cell marking with easily detectable marker genes such as fluorescent proteins (FP) can be performed by using integrating vector systems. However, the number of available FP is limited and only a very small number of individu…