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Cysteine Proteases in acute myeloid leukaemia

S Prall, J Fisher, F Pontan, O Heidenreich · Klinische Pädiatrie · 2011 · Heft 05

Dokument
866526
CareLit-ID
Jahr
2011
Publikation
PDF
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Metadaten
DOI
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Bibliografische Angaben

Zeitschrift
Klinische Pädiatrie
Autor:innen
S Prall, J Fisher, F Pontan, O Heidenreich
Ausgabe
Heft 05 / 2011
Seiten
–
Erschienen: 2011-05-05 00:00:00
ISSN
0300-8630
DOI
10.1055/s-0031-1277071

Zusammenfassung

We have previously identified calpains as important modulators of AML1/MTG8-dependent leukaemogenesis. Like caspases and cathepsins, calpains belong to the family of cysteine proteases. By application of cysteine protease inhibitors and siRNAs we investigated individual cysteine proteases essential for leukaemic growth, maintenance and progression. Cells were transfected with siRNAs using electroporation; knockdown was confirmed by real-time RT-PCR. Cysteine protease inhibitors were applied in various concentrations. Proliferation was determined by cytotoxicity assays and cell cycle analysis. Clonogenicity was a…

Schlagworte

Cysteine Cysteine Proteases Cathepsins Cathepsin B Caspases Calpain Klinische Pädiatrie