Effects of GLP-1 incretin – role of the GLP-1 metabolite (GLP-1 (9–36) amide)

Aims: In our preceding study DPP-4 inhibition diminished GLP-1 incretin effects. This finding sheds new light on biological role of GLP-1 metabolite (GLP-1m). In the present study we investigated the involvement of GLP-1m in the effects exerted by GLP-1 incretin and the time duration through which GLP-1m effects are maintained. Materials and methods: (A) Chronically catheterized Wistar rats were injected with vehicle (V; 0.1% bovine serum albumin in saline), 4.0 nmol/kg GLP-1m (Probiodrug AG, Halle/Saale, Germany), 4.0 nmol/kg GLP-1a (GLP-1 (7–36) amide; Neo MPS, Strasbourg, France) or GLP-1m and GLP-1a each at…