SGBS cell differentiation – a model for adipogenesis – studied by quantitative proteomics and metabolomics
C Küntzel, D Friebe, K Landgraf, S Kalkhof, S Baumann, W Kiess, M von Bergen, A Körner · Diabetologie und Stoffwechsel · 2011 · Heft 08
Bibliografische Angaben
Zusammenfassung
Introduction: The Simpson-Golabi-Behmel Syndrom (SGBS) cells are a reliable model to study human adipogenesis. However, the molecular mechanisms that occur during adipocyte differentiation and which are potentially involved in the development of obesity and related sequelae are not fully understood. Therefore the aim of this study was to investigate comprehensively the adipogenesis of SGBS preadipocytes to adipocytes on a molecular level using a global proteomics and a targeted metabolomic approach. Methods: In this study we used Stable Isotope Labeling in Cell Culture (SILAC) combined with LC-MS/MS for an accur…
