Identification of Cancer Stem Cells in Primary Ovarian Carcinoma

Fragestellung: In a wide range of tumor entities cancer stem cells were identified as subpopulation with a higher tumorigenic potential than the bulk of tumor cells. The aim of this study is to find cancer stem cells in the primary ovarian carcinoma and to study differences in molecular and functional biology. Methode: Tumor samples were taken from primary ovarian carcinoma and a protocol based on collagenase digestion to disrupt the tissue into single cell suspensions was developed using the Miltenyi Gentle MACS Dissociator®. Single cell suspensions were analyzed for viability and short term cell culture were e…