Molecular analysis of MTSS1 in gliomas
D Luxen, M Gessi, LJ Isselstein, T Müller, D Freihoff, J Freihoff, A Becker, M Simon, T Pietsch, A Waha · Klinische Pädiatrie · 2012 · Heft 04
Bibliografische Angaben
Zusammenfassung
In a genome-wide methylation analysis, the metastasis suppressor-1 (MTSS1) gene was identified as a novel gene hypermethylated in gliomas. MTSS1 interacts with actin filaments and promotes Gli dependent transcriptional regulation. Here we investigate MTSS1 methylation, allelic loss and expression in 63 gliomas and 6 glioma cell lines by pyrosequencing, realtime RT-PCR and IHC. MTSS1 was overexpressed in glioma cell lines to study localization and biological function of the protein. MTSS1 hypermethylation was frequently detected in anaplastic astrocytomas and secondary GBM where it correlated with IDH1 mutational…
