Pulmonary Haptoglobin (pHp): a candidate gene and its validation
M Abdullah, D Kähler, S Marwitz, C Vock, N Reiling, C Kugler, H Fehrenbach, F Pedersen, H Watz, KF Rabe, P Zabel, E Vollmer, K Dalhoff, T Goldmann · Pneumologie · 2012 · Heft 05
Bibliografische Angaben
Zusammenfassung
Haptoglobin is a long known acute phase protein involved in hemoglobin breakdown, which was thought to be from the liver. The discovery of pHp was achieved by applying transcriptome analysis to human lung tissues which were subjected to a short term culture and infected or not infected with Haemophilus influenzae. These tissues were fixed and paraffin-embedded by the HOPE-technique. First, RT-PCR and RNA-in situ hybridization was used to confirm the pulmonary derivation of pHp. Immunohistochemistry was performed and showed expression of haptoglobin in Alveolar Epithelial Cells type II (AECII), macrophages (MA),…
