IRF3 KO mice are protected from polymicrobial sepsis by an MIP-2 dependent mechanism

Introduction: Interferon regulatory factors (IRFs) play diverse roles in innate and adaptive immunity by promoting type I interferon production and regulating the function of interferon-induced genes that are implicated in chemokine or cytokine production. We have previously reported that IRF3 but not IRF7 deficiency protects mice from bacterial-induced sepsis. Methods: In the present study, we aimed at investigating the underlying mechanisms protecting IRF3-/- mice using real-time PCR, ELISA, multiplexed flow cytometry chemokine detection and immunohistochemistry. Results and discussion: Splenocytes from IRF3-/…