In vitro and in vivo models for irinotecan induced steatohepatitis

Irinotecan induced steatohepatitis is a risk for perioperative morbidity and mortality, however, the underlying mechanisms are still unknown. The aim of this study was to establish in vitro and in vivo models of for irinotecan induced steatohepatitis to unravel the molecular mechanisms of this phenomenon. Methods and Results: Initially, we determined the dose-range in which irinotecan treatment did not affect the viability of hepatoma cells and primary human hepatocytes. In this dose-range (of up to 50µM) irinotecan induced a dose-dependent lipid accumulation as assessed by oil-red o staining and analysis of the…