Hematopoietic-specific lyn-substrate 1 (HCLS) is deacetylated by NAMPT/NAD+/SIRT1 pathway

Recently we identified HCLS1 protein to be an essential player in the G-CSFR signalling pathway in myeloid differentiation (Skokowa et al., 2012). We also described a new G-CSFR signalling through activation of Nicotinamide phosphoribosyltransferase (NAMPT)/NAD+/Sirtuins in healthy individuals and in CN patients. We found that G-CSF-triggered deacetylation of myeloid specific factors is essential for myeloid differentiation (Skokowa et al., 2009). In the present study we aimed to investigate whether HCLS1 is regulated by NAMPT/NAD+/Sirtuin pathway via deacetylation. We identified four acetyl-lysine sites in HCLS…