Acetylation of LEF-1 transcription factor regulating hematopoietic differentiation

Previously we identified that NAMPT is essential for the G-CSF-triggered granulocytic differentiation via a NAD+- sirtuin-1-dependent pathway. Sirtuins are NAD+-dependent protein deacetylases and we found that myeloid-specific transcription factors C/EBPα and C/EBPß are deacetylated and activated by SIRT1. We and others demonstrated, that lymphoid enhancer-binding factor 1 (LEF-1) transcription factor is crucial for neutrophil granulocytopoiesis and lymphopoiesis. LEF-1 mediates the proliferation, survival and differentiation of granulocyte and lymphocyte progenitor cells by binding and activation of cell type-s…