Hepatic differentiation of hiPSC in a 3D multicompartment bioreactor

The hepatic differentiation of human induced pluripotent stem cells (hiPSC) holds great potential for applications in regenerative medicine, pharmacological drug screening and toxicity testing. To date hiPSC derived hepatocytes show characteristics of a fetal state but the maturation has to be further improved. Perfused 3D hollow fiber membrane bioreactors provide a more physiological environment compared to open static 2D cultures and we hypothesize that the usage of such a 3D culture system could improve the hepatic differentiation of hiPSC. Here, we describe the hepatic differentiation of hiPSC within a 3D mu…