Correlative three-dimensional observation of lung tissue by different tomographic methods

Combining light- and electron optical methods on the same sample facilitates the understanding of organ structure by providing both overviews of large samples as well as high resolution insights into very small structural features. We present here the combination of Scanning Laser Optical Tomography (SLOT) with Electron Tomography (ET) to examine mouse lung tissue at low and high magnifications. SLOT allows to analyze, reconstruct and segment a whole mouse lung with a resolution allowing the recognition of structures in size down to single alveoli. The resulting three-dimensional models allow for example the det…