Genome-wide microarray-based screen for FOXJ1-dependent ciliary factors in the murine lung

Ciliogenesis is crucial for proper organogenesis during mouse embryonic development and homoeostasis of adult tissues. A key regulator of motile cilium formation is the transcription factor FOXJ1 that directs ciliogenesis in various tissues including the respiratory epithelium of the lung and the ependymal epithelium of the brain. Downstream effectors of FOXJ1 are only partially known. In order to elucidate processes downstream of FOXJ1 that initiate ciliogenesis or enable cilium function, we conducted microarray screens comparing the murine transcriptomes of (1) Foxj1-deficient and wildtype lungs at embryonic d…