Cytotoxicity of anisidine in hepatic cell lines

Humans are exposed to azo dyes and its degradation products like aromatic amines through different consumer product. The ECHA classified the aromatic amine o-anisidine as carcinogen (Carc. 1B) and mutagen (Muta 2) due to increased rates of bladder and kidney tumors in mice and rats. In contrast, p-anisidine was classified with specific organ toxicity (STOT RE 2) only. Hepatotoxic effects of o- and p-anisidine were studied using HepG2 and Huh-7 cells. Cells were grown in DMEM (Huh-7) or RPMI medium (HepG2), supplemented with 10% FCS, 5 mM L-glutamine, 100 U/ml penicillin and 0.1 mg/ml streptomycin. EC50 values we…