Xenobiotic-dependent induction of the tricarboxylate carrier Slc13a5 in primary cultures of rat hepatocytes

Background: The tricarboxylate carrier Slc13a5 (mINDY) is an active transporter that mediates concentrative uptake of citrate in symport with sodium ions across the plasma membrane of cells. In mammals, it is expressed predominantly in hepatocytes and appears to contribute to the cytosolic citrate homeostasis. Citrate is an important intermediate and regulator of fatty acid synthesis. Accordingly, knock down of the Slc13a5 gene in mice was associated with reduced liver fat content and consequently improved insulin sensitivity under a high fat diet. Exposure to xenobiotics often results in hepatic lipid accumulat…