Liver inflammation abrogates T cell mediated tolerance against particulate antigens induced by liver Kupffer cells
F Heymann, J Peusquens, I Ludwig-Portugall, M Kohlhepp, C Ergen, P Niemietz, C Martin, N van Roojen, J Ochando, G Randolph, T Luedde, F Ginhoux, C Kurts, C Trautwein, F Tacke · Zeitschrift für Gastroenterologie · 2015 · Heft 01
Bibliografische Angaben
Zusammenfassung
Background and Aims: Liver Kupffer cells are key players in the upkeep of body homeostasis, ingesting apoptotic material, gut derived microbiota and particulate antigens from the circulation. We investigated the immunoregulatory capacities of liver phagocytes in healthy and inflamed conditions against particle bound antigens addressing T cell mediated immune tolerance. Methods: 0.5 µm fluorescent latex (Lx) particles were covalently linked to ovalbumin (OVA) and injected i.v. into recipient mice. Particle distribution was investigated in CX3CR1.eGFP and wildtype animals by flow cytometry and multiphoton microsco…
