Optical imaging using an Hsp70 NIR probe to monitor the therapeutic efficiency of hyperthermia in vitro and in vivo

Zielsetzung: Hyperthermia as cancer treatment is capable to induce cell death and consequently stop tumor growth. Tumor cells are specifically prone to hyperthermic temperatures; therefore, heat shock proteins (Hsp) like Hsp70 are expressed upon cellular stress. Here, an optical near-infrared (NIR) probe was developed to assess the therapeutic efficiency of magnetic hyperthermia on a molecular level in breast cancer. Material und Methodik: The Hsp70 antibody was covalently coupled to the fluorophore Dy700. The breast cancer cell line MDA-MB-231 was used for immunofluorescence, affinity studies and FACS analysis.…