Normalization and calibration in the metagenomic analysis of CF lung microbiomes

Next-generation sequencing is becoming increasingly affordable, which makes whole-metagenome sequencing an attractive alternative to traditional 16S rDNA, RFLP, or culturing approaches to analysis of metagenomic samples. The advantage of whole-metagenome sequencing is a broader spectrum of detectable microorganisms (including also fungi and many viruses), quantitative analysis of the microbial community and information about the metabolic capacity and physiological features of the studied metagenome, even without the knowledge of genotypes and phenotypes of all members of the microbial community. Problems of 16S…