Intravital real time imaging of liver damage and regeneration by functional two-photon microscopy

Recently, we have established an intravital two-photon based imaging technique that allows real-time observation of the living mouse liver. Videos can be taken at subcellular resolution (200nm) but also the imaging of overviews over several lobules is possible. This technique gave us already surprising insight into liver disease and hepatotoxicity. In this presentation, videos will be shown illustrating the morphological key events how paracetamol (APAP) damages liver tissue. The earliest event, approximately 20 min after APAP injection, was loss of mitochondrial activity of pericentral hepatocytes as evidenced…