Inactivation of fatty acid synthase impairs hepatocarcinogenesis driven by AKT in mice
DF Calvisi, L Li, GM Pilo, A Cigliano, S Ribback, F Dombrowski, X Chen, M Evert · Zeitschrift für Gastroenterologie · 2015 · Heft 12
Bibliografische Angaben
Zusammenfassung
Background: Cumulating evidence underlines the crucial role of aberrant lipid biosyntesis in human hepatocellular carcinoma (HCC). Here, we investigated the oncogenic potential of fatty acid synthase (FASN), the master regulator of de novo lipogenesis, in the mouse liver. Methods: FASN was overexpressed in the mouse liver, either alone or in combination with activated N-Ras, c-Met, or SCD1, via hydrodynamic injection. Activated AKT was overexpressed via hydrodynamic injection in livers of conditional FASN or Rictor knockout mice. FASN was suppressed in human hepatoma cell lines via a chemical inhibitor and speci…
