Beta Adrenergic receptors stimulation and role of NF-κB and IκBα in hyperglycemic endothelial cells

Background: Apoptosis and inflammation are important features of endothelial dysfunction in diabetes. NF-κB plays a key role in inflammation and apoptosis through its ability to induce transcription of pro-inflammatory genes. In this study, we investigated the effect of β-adrenergic receptor stimulation on NF-κB and IκBα mediated apoptosis, inflammatory cytokines and adhesion molecules in hyperglycaemic HUVECs. Methods: Human umbilical vein endothelial cells (HUVECs) were cultured in high (25 mM) and low (5 mM) concentrations of glucose. Cells were treated with 5, 10 and 20µM isoproterenol and propranolol for 6,…