Differences on hepatic Igfbp2 methylation and expression occur before the onset of fatty liver disease

Background: NAFLD patients exhibit decreased hepatic Insulin-like growth factor binding protein-2 (IGFBP2) expression associated with decreased DNA methylation1. Transcriptome analysis of livers from adiposity-susceptible (responder) C57BL/6J mice showed also decreased expression levels of hepatic Igfbp2 compared to adiposity-resistant (non-responder) mice. The aim of this study was to test if hepatic DNA methylation and Igfbp2 expression is altered before onset of hepatosteatosis. Methods: Male C57BL/6J mice on high-fat diet were grouped into responder or non-responder using previously established prediction cr…