Systemic characterization of macrophage phenotypes in allergic airway inflammation

Introduction: Macrophages are central players in lung pathology with both regulatory and effector properties. Besides activation toward the M1 subtype (classic activation), alternatively activated M2 macrophages have been implicated in pneumonia and allergy. We investigate the role of microRNAs in macrophage polarization-associated diseases. Here, we analyzed the systemic RNA phenotype of murine lung macrophages in allergic airway inflammation. We are establishing an in vitro model with bone-marrow-derived macrophages (BMMs) to mimick the transcriptome changes which we observed here. Methods: Macrophages from th…