Endogenous intestinal alkaline phosphatase controls inflammatory pathways and cellular homeostasis in the gut

Objective: The inability of epithelial cells to cope with various stresses and cellular damage plays a crucial role in the development of many diseases in the gut such as colitis, Crohn's disease and carcinoma. The exact functions of alkaline phosphatases in various cellular pathways are not well understood. We propose that intestinal alkaline phosphatase (IAP) plays a critical role in intracellular homeostasis and stress responses in the gut. Methods: IAP protein complexes were immuno-precipitated using Anti-RFP antibody followed by masses identification using liquid chromatography-tandem mass spectrometry (LC-…