Follicle proliferation in cryopreserved human ovarian xenotransplants

Objective: To investigate follicle proliferation capability of cryopreserved human ovarian grafts transplanted into the chick embryo chorioallantoic membrane (CAM), in order to better understand early follicle development after ovarian cryopreservation using a low-cost, minimally invasive animal model. Materials and methods: Forty nine frozen ovarian tissue samples obtained at Universitätklinikum Ulm from two women undergoing laparoscopic surgery to cryopreserve ovarian tissue for fertility preservation purposes were thawed and transplanted on day 5 fertilized eggs from White Leghorn chickens, with and without F…