Alcohol-related vitamin A hepatotoxicity is mediated by polar retinoid metabolites and involves apoptosis
D Zili, Y Popov, E Patsenker, C Liu, XD Wang, HK Seitz, D Schuppan, F Stickel · Zeitschrift für Gastroenterologie · 2004 · Heft 02
Bibliografische Angaben
Zusammenfassung
Introduction: Vitamin A hepatotoxicity may be markedly increased by concomitant alcohol consumption but the mechanisms of this interaction are poorly understood. Alcohol-induced hepatic microsomal cytochrome P4502E1 metabolizes retinoids into polar metabolites which may be responsible for the observed liver damage. However, direct experimental evidence supporting this hypothesis is still lacking. Aim: To investigate the effects of polar retinoid metabolites (PRM) on the viability of cultured HepG2 cells and freshly isolated rat hepatocytes. Methods: PRM were extracted by HPLC from liver microsomes of Sprague-Daw…
