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Activation of Caspases in HepG2 Cells is Dispensable for Cytokine-mediated Apoptosis Induced by either TNF, TRAIL or Agonistic anti-CD95 Antibody

G Dünstl, G Künstle, C Schlaeger, T Meergans, A Wendel · Zeitschrift für Gastroenterologie · 2004 · Heft 02

Dokument
908490
CareLit-ID
Jahr
2004
Publikation
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DOI
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Bibliografische Angaben

Zeitschrift
Zeitschrift für Gastroenterologie
Autor:innen
G Dünstl, G Künstle, C Schlaeger, T Meergans, A Wendel
Ausgabe
Heft 02 / 2004
Seiten
–
Erschienen: 2004-02-02 00:00:00
ISSN
0044-2771
DOI
10.1055/s-2004-816172

Zusammenfassung

Actinomycin D-sensitized HepG2 cells are highly susceptible to induction of apoptosis via stimulation of death receptors (TNF-R1, CD95 and TRAIL receptor). We showed that the pan-caspase inhibitor zVADfmk conferred complete protection of HepG2 cells against apoptosis induced by TNFalpha or agonistic anti-CD95 antibody 1. Here we provide evidence that prevention of apoptosis by zVADfmk is not due to inhibition of caspases but rather due to inhibition of a yet unidentified target. Incubation of HepG2 cells with high concentrations of the pan-caspase inhibitor zVADfmk conferred complete protection against TNF-, TRA…

Schlagworte

Apoptosis Caspases Death Receptor HepG2 Cytokine Cathepsins Actinomycin D Actinomycin Zeitschrift für Gastroenterologie