Three-dimensional reconstruction and analysis of gastric biopsy specimens by electronic slides of consecutive sections and virtual microscopy

Background: The application of an electric slide and a software simulated virtual microscope can contribute to a more efficient and convenient histological objects. Material and methods: From routine gastrointestinal biopsy specimens 5 micron thick, H/E stained, 50 to 300 serial sections were prepared and barcode labeled. A fully computer controlled scanning microscope was used for the scanning. A program called 3D-SCOPE was developed for the virtual microscopy orientation, analysis of the sections. Using the mathematical modules for automated surface epithelia, gland, non-epithelial tissue determinations, tissu…