Isolation and Purification of Primary Human Liver Cells – Impact of Liver Pathology on the Outcome

Introduction: Large quantities of primary human hepatocytes are required for in vitro models enabling the investigation of metabolism and cytotoxicity of xenobiotics as well as studies in the field of regenerative medicine. Since availability is still scarce, liver samples from resected liver specimen are used as one possible cell source although isolation outcome is often unsatisfying. Aim: We analyzed the impact of liver pathology on the outcome of the isolation and purification of liver cells obtained from resected liver specimen. Method: Samples were taken from patients undergoing partial hepatectomy for var…