Hybrid-primed lymphocytes and hybrid vaccination prevent tumor growth of lewis lung carcinoma in mice

Dendritic cell (DC)-tumor cell hybrids are currently being evaluated as a novel anti-tumor vaccination strategy. We here explored in an animal model whether administration of DCs fused with poorly immunogenic carcinoma cells could elicit an anti-tumor response. Fusion of C57BL/6 mice bone marrow derived DCs with Lewis Lung Carcinoma (LLC1) cells resulted in around 50% fusion efficiency. Hybrid cells (HC) were used to explore three potential tumor-therapy strategies: protective immunization, vaccination and adoptive cellular therapy. Immunization with HCs induced activation of proliferating and cytotoxic T cells…