Altered beta 1 integrin expression in renal cell carcinoma exposed to valproic acid – disease progression and new theraputic options

Aim: To elucidate the role of beta1 integrin expression during progression of renal cell carcinoma (RCC). Materials and methods: The experiments were based on Caki-I and KTC-26 kidney carcinoma cell lines and normal renal proximal tubular epithelial cells (PTC). Alpha 1 to alpha 6 beta 1 integrin subunits were evaluated by RT-PCR and Western blot, and integrin surface expression was analyzed by flow cytometry and confocal microscopy. The expression pattern was correlated to tumor cell growth and adhesion to an endothelial cell monolayer. In order to examine the relevance of integrin alterations on their malignan…