Differentially expressed microRNAs during myofibroblastic transdifferentiation of Hepatic Stellate Cells
I Strack, M Scheffler, S Schievenbusch, J Riemer, A Noetel, U Töx, M Kwiecinski, N Elfimova, I Wedemeyer, HP Dienes · Zeitschrift für Gastroenterologie · 2009 · Heft 01
Bibliografische Angaben
Zusammenfassung
Aims: Activated Hepatic Stellate Cells (HSC) play a crucial role in hepatic fibrogenesis. MicroRNAs (miRNAs) are short regulatory RNA that result in posttranscriptional gene repression and are involved in cell differentiation and carcinogenesis. In the present study we analysed the function of miRNA in myofibroblastic transition of HSC and liver fibrogenesis. Methods: Total RNA was extracted and a miRNA expression profile was analysed by microarray hybridisation and real-time PCR assays of total RNA extracts from primary rat HSC. miR-125b was then either knocked out or elevated in primary HSC by transfection of…
