Proteomic analysis and 2-D-Gel electrophoresis of bile-duct bile: A new diagnostic approach

Aims: Extensive research has been performed on the lipid composition of bile and its mucin content, but not on differences in protein composition. Though this could identify new molecular players and markers of disease. Methods: Bile samples were acquired by ERC from patients suffering from gallstones, primary sclerosing cholangitis (PSC), liver transplantation (LTx), and/or malignancy of the pancreatico-biliary tract. Purified protein samples were analyzed by 1- and 2-D-Gel electrophoresis, DIGE and mass spectrometry. Results: We collected >800 bile samples from different cholestatic diseases. A new purificatio…